FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740283
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2800.048.5452887
CACGTCT3550.036.274029
ACACGTC3650.035.2802128
GCACACG5200.026.139776
AGCACAC5300.025.6465685
AGAGCAC5900.023.043113
GAGCACA6200.021.9236774
CTACACG3100.00616255413.919871
AAGAGCA11500.013.0700842
AGTCACT2404.0017767E-1111.3622320-24
GAAGAGC12054.1836756E-1111.3399771
CCTCAAA8000.00477332348.0909251
GGGGGGG2151.1677321E-48.062036100-104
GTCACTG3802.3355824E-97.93152920-24
GAGAGTA3701.2234523E-87.74707730-34
CCAGTCA4601.6898412E-97.176145620-24
TCACTGG3852.0123116E-77.07221525-29
AATCTTT12406.726977E-46.9232675
CCTCCGC10800.009112756.65919731
CAGTCAC4157.212566E-76.57092820-24
Produced by FastQC (version 0.11.5)