FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647493
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAC3200.00727193213.5757951
AGGGTAA3900.00183048112.9504695
CACCAAT6202.4057645E-612.8459151
CTCAAAT5351.1178125E-412.1801541
CTCAAAA8403.90246E-812.0673751
CATTTCG6556.155842E-511.0156665
ACCAATC4800.00861379610.5255072
CCCAGAT4850.00908523910.4500971
CTTATAA8456.6204957E-610.282261
CTTATTC5950.0036853299.7350251
CCTGTAT6300.00590418379.194191
CATCGTT6600.0086485838.7762721
TTTAAAG8100.00516825778.0194342
GTTTCAT9400.0022092787.70257951
ATTTCGG9550.00267016587.5552486
CTTTTAT13401.8509217E-47.02429251
CAAAGAA15350.0064638085.6602671
CTTTTTT25652.2456783E-55.36327741
Produced by FastQC (version 0.11.5)