FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937375
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3158.971565E-720.2686657
ACACGTC4102.7801434E-819.0327728
CACGTCT3856.103546E-616.5834529
GCACACG6401.9106665E-814.4097556
GAGCACA6402.9496914E-713.31267454
AGCACAC7001.17163945E-511.1477665
CCCAAAG6608.5433654E-49.7780081
AGAGCAC6700.00107437289.5364213
ATTTAAC9100.00196534237.7956416
ACTGCCG11100.00174454697.0301237
CGATGAC11900.00324199446.62822961
AAGAGCA12600.00653164836.19783262