FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2630814
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT10158.936695E-910.8108117
CACGTCT10501.5159458E-810.4504519
ACACGTC10008.3022314E-810.2871618
AGCACAC16301.5866626E-67.1523565
GAGCACA15354.962212E-67.1489244
AGAGCAC14551.8024088E-57.06994533
CCCATGT15504.2243526E-56.6514181
GCACACG14951.9513347E-46.42206436
CCCACAC14850.00121886576.01688341
TCAACTT19650.00110343585.23499773
GTCAAGA19850.00122590265.1938031
CCCAAAG22552.2471455E-45.1815191
GTTTTAT33552.9850344E-75.1215591
CTGGAAT30105.467027E-65.0235521
Produced by FastQC (version 0.11.5)