FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008750

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008750
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1586532
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAT5404.586218E-714.8847331
CTCAATT10558.180493E-58.31133651
CTCCTCT11251.7264717E-47.7941871
TTGCGTA9550.0026791647.5541528
CCCATAT13552.330707E-57.54972031
CTTAGAA10050.00389360757.2706971
CCCTTGT11200.00140403187.17656761
GTTCAGT10300.0049459627.09422351
CTTTGAT29601.4551915E-116.9120751
CTTCTAT17551.411383E-56.66169831
CCTCGAT12550.00464698676.4045861
CTTAAAG14000.00209651846.2631861
TCGCTGA13950.0023250146.2057768
CTCAAAG16904.185061E-46.0531781
CTTTTAT34105.14774E-105.9999241
CTTTAAC14650.00348526665.98529671
CTCATTT15950.00137698435.95558931
CTCAAAT14750.0037599765.94471931
CTCCTTT29209.554096E-75.50531241
CTTAAAT15950.0089395985.4974671
Produced by FastQC (version 0.11.5)