FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323675
Sequences flagged as poor quality0
Sequence length100-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT4007.686198E-616.1870217
CACGTCT4804.3037573E-513.4891849
AGCACAC8004.384424E-59.8924435
ACACGTC6608.293737E-49.8103168
GAGCACA7703.252411E-49.34386354
AGAGCAC9650.00304542237.4557263
CTTTTAT14203.8868823E-46.6124961
AAGAGCA16303.1966512E-46.18096262
TTCGAAC14950.00517334845.77637962
Produced by FastQC (version 0.11.5)