FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1203601
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAA5455.998263E-714.5350111
CACACGT3800.001542030513.2503747
CCCAGGG3900.00185868812.9256561
CACGTCT4050.002490412412.4319339
ACACGTC4500.005472916611.1892058
CCCCGAT7101.4238602E-410.142871
GAGCACA6800.00109002539.5218094
CCCAAAT7803.6665594E-49.2326121
AGCACAC7100.00161659629.1194795
AAACTCG7100.00161659629.1194795
CTACCGC8650.00102752968.3253611
CCCATAT7800.00375815368.3093511
CCTCCAT11402.667425E-58.2121651
CTCTATT8200.0058944557.90401651
TCGCCCG12609.421009E-57.4211039
CTACAAA11753.3715615E-47.35465961
CCTTGAT16651.0226559E-67.3528181
CTTTTAT24951.546141E-107.2158691
CAACACC14406.3052954E-57.0013971
CTTCCAT21702.8272552E-86.96913241
Produced by FastQC (version 0.11.5)