FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161612
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2401.680326E-624.0283477
ACACGTC3453.8651597E-516.715378
CACGTCT3707.032723E-515.5859539
CTAGAAT3150.006530790613.7993851
GCACACG5951.595703E-613.3266466
GAGCACA6204.5740558E-410.4657584
AGCACAC8104.9150687E-59.7897475
AGAGCAC6900.00122134279.4040153
CTTAAAC8700.00102519038.3272151
TATACCT10300.0056405796.99854767
CCCAGAA10650.0074271736.80251361
CAAGATA15551.6952615E-46.49108844
TTGATTC13850.0021581416.24671033
CCCTTTT16603.7082023E-46.10996871
CTTAAAT15750.00131082775.9797331
TACCTTT15800.0093491785.47481259
TTATTAC19806.2539993E-45.4609876
CTGAAAT20157.398408E-45.39305971
Produced by FastQC (version 0.11.5)