FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4078026
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAAT47650.07.36643171
CCCCCGA13702.1597663E-46.9390751
CTCCGAT35150.06.86542461
CCTAATT13000.00665672876.18768451
CTCACGA16553.1694208E-46.18598461
CTTCAAT66600.06.1488321
CCCACTT20251.7174962E-56.13907151
CCTCAGA15550.0010068496.1135261
CCTTAAT20602.2352731E-56.03476761
CCCACAA28257.976996E-85.9537171
CCCACTC15200.0052123365.7731981
CAACGCT25653.1762593E-65.70192431
CTCACAA21905.6914563E-55.6765391
CTTAAAA47601.8189894E-125.5306271
CCCAAAC22508.5718E-55.52516461
CTCATAT33557.894414E-85.44911861
CCTATTA20659.086889E-45.31190161
CTCAAAT33103.4120967E-75.3022731
CTTCGAT44303.5106495E-105.28232431
CCCTCAT22154.060035E-45.28232431
Produced by FastQC (version 0.11.5)