FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2379314
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT8104.3747787E-711.3389049
CACACGT8458.898764E-610.033157
ACACGTC10401.02590595E-48.1519358
GCACACG14401.0248959E-57.35953956
GAGCACA16959.932287E-56.25261554
AGCACAC13650.00230290936.2113895
CTCAGAT15050.0010138366.10972741
AGAGCAC16300.00266593675.6350253
CTTTAAT26857.0927636E-55.00523331
Produced by FastQC (version 0.11.5)