FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107725
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA3504.306972E-516.5054349
CTTGAGT4050.002234739312.6124711
GCCACTC4907.4815325E-411.7895968
AGCCACT5551.6153025E-411.7099367
AAGCCAC8750.00112052688.2527166
GTATAAT10659.268721E-58.2222151
CTCGGAA8250.0055279947.96059851
CCTCAAC8700.0088943487.54884341
CTTTCAT16757.2079147E-66.9704741
CCTCGAT12606.4487546E-46.94972851
CTTTAAA12656.746817E-46.92225931
CTTCTGC21301.1651355E-76.85184531
CTTGAAC11900.00269869536.74532561
CTCCTTT19651.561626E-66.68447161
CTCGAAG11000.0094627366.63383251
ATTTTAC12150.00374621276.5376466
CTTTCAG20854.1248823E-66.29975371
CATATTG15007.5762527E-46.258315
CTTCGGG14000.00212684466.2547561
CAAAGAA24802.765646E-76.17909341