FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036594
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCAT7502.0621268E-510.59318
CTCGCTT5600.002036912410.4576981
CTTGGAT6758.686379E-49.7605191
CCCTGTT8054.2660852E-49.0936521
CTTAAGA6550.00743280078.9409331
CTCATAG6650.008415598.8064831
CTCCACA9853.4872827E-48.1750541
CCTCGAT8300.0056717537.9377721
CAATTGA9650.00293137627.48452574
CTTCTTT34450.07.43726061
TTTAGCA11953.9559402E-47.25278767
AATTAAC10100.0045735157.1510576
CCCAGCT10250.0046311257.14184331
CATTCAT12354.945037E-47.11292841
CTTCAAA19651.9726576E-77.0782391
CTTCCAT18807.1345494E-77.00888351
CTTTAAT20253.3315882E-76.86851361
CACAGAT10700.00702635256.8414851
GTTCAAT10700.00702635256.8414851
GACAAGA11700.00251310046.7911032
Produced by FastQC (version 0.11.5)