FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160641
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCACT4500.005304757511.2361181
CTACGAA6658.5645914E-49.77578451
CCCAAGG8609.260325E-59.2390011
CTCAGCT6350.0064314599.1001191
CTTTAAT12851.5365913E-78.9938911
CTTCACA8407.4528233E-48.5990711
CTCATAA10559.352346E-58.2159841
CCAATTT9854.1646106E-48.0415073
CTCCATT10851.2960237E-47.98881341
CCTTCGA8350.00674919747.7855061
CTCAAGC11452.4170427E-47.5701851
CAACGAT11803.4190965E-47.34564641
CTTCAAT15201.602991E-57.1281761
CTCATAG10400.00607334876.94540261
CTTCAAA20354.5803063E-76.7440371
CAACGGT11900.0030039576.67692471
CTTCTAT10950.0099915056.59654661
CACCGCT12050.00342560196.593811
CTTCTGT13150.00117713986.59153031
GATCTTC13150.00121872136.5713592
Produced by FastQC (version 0.11.5)