FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431297
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAAT8254.1682324E-711.380436
ACTTTAG4900.00999687710.3174245
CAAGGCC9653.2360124E-48.2325559
CCCCGAA8000.00442383078.1606081
CTTTGAT27653.8388862E-75.77161741
ATTTACC17750.00562128475.2894966
CCAAAAT20609.782182E-45.2819471
TTCAAAC17800.00576478855.27814152
Produced by FastQC (version 0.11.5)