FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581649
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGGGA3100.00618205913.9125324
GGATTGT3700.001266309213.5991874
CATAAAC6657.1511306E-510.8540361
TTAATCT5300.0014971410.8500253
CAAAGCT8709.716152E-69.9557711
GTTCCTT7300.0020169248.8988231
CTTTCAT9300.0020498457.76121951
CCCAGTA2700.00142890216.480405120-124
CTTCTTC15650.0082782125.53451871
Produced by FastQC (version 0.11.5)