FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421232
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT5603.5342964E-916.3747717
CACGTCT5758.654388E-814.7208639
ACACGTC7409.604264E-913.3449288
GCACACG10851.8097126E-79.7513816
GAGCACA9654.2218926E-69.5024574
CTCCAAT10551.2512155E-69.38353351
AGAGCAC11755.555041E-68.404763
AGCACAC10751.534633E-47.8736735
ATTACAT11600.00294915966.6888987
AATTACA12250.0052172936.3337546
ATATCGA13400.00190530616.31656656
GGAGAAT18200.0016330275.4393561
TGATTTT33852.066663E-75.20956234
CCTTGAT20650.00141915225.13643741
AAGAGCA19250.0033846195.1323452
GAAGAGC18150.0093359925.06474451
TTGATTT28002.746601E-55.0385683
Produced by FastQC (version 0.11.5)