FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429318
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1703.1263462E-629.7034828
CACACGT1804.878584E-628.0532887
CACGTCT1959.086447E-625.8953449
AGCACAC3101.5282803E-518.6160075
ACGATCG2450.001256989217.6662084
GCACACG2901.9350933E-417.4123866
AGAGCAC3554.8852133E-516.260023
GAGCACA3406.497547E-414.8517414
ATTAAAG4150.002907687812.174782
GAAGAGC5551.5302416E-411.77469351
ACTATCG1450.00189032699.9499359
Produced by FastQC (version 0.11.5)