FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629418
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT5206.184564E-1119.0448639
ACACGTC5401.07320375E-1018.3394998
CACACGT5601.7826096E-1017.6845177
AGCACAC10801.2187229E-911.1370885
GCACACG9954.884714E-810.66533856
AGAGCAC10601.2320743E-710.0122543
GAGCACA11202.7464557E-79.47588354
CCCGCTT12551.3835343E-68.4716461
CTCCGTT13453.7214104E-67.90477041
CTTAAAG13604.356987E-67.81758551
CCCCGAA10307.963571E-47.5696491
CTCGAAA10509.775162E-47.4254651
CCTCAAT12306.833225E-46.9150671
CTCAAGA10300.00664575446.88149931
CTCCCAT11950.00382882266.5244671
CTGAAAT26204.7457434E-96.4927731
CTCCGAA12050.0041773286.47032261
CCAAGCT13250.00158908136.41926961
CTCCAAA15502.0302873E-46.4020141
CTCCAAT13300.00165804026.3951381
Produced by FastQC (version 0.11.5)