FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences882895
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT3602.2191671E-1023.8159167
AGCACAC4302.0390871E-919.938915
GAGCACA5053.45608E-1119.8072994
CACGTCT4854.3837645E-1019.1509449
ACACGTC5607.2759576E-1219.137798
AGAGCAC5051.495573E-816.9776843
GCACACG5951.1224802E-714.4096316
CTTAAAC3100.00622640913.8986531
AATTAAG4250.003755451411.7678647
AAGAGCA9402.7142289E-59.1297812
GTGATAC6600.009257848.7042071
GAAGAGC11101.8052098E-47.7632121
ATTTTAC9850.00397999177.25357965
CTTTATT14750.0045574165.8421461
CTTTCAG18400.00154084235.46378231
CTTTTCA17650.00561857135.28911
Produced by FastQC (version 0.11.5)