FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811605
Sequences flagged as poor quality0
Sequence length100-151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGAA4650.006644300710.9006221
TGGCAGC5350.00156458110.7938068
CTCCAAC5950.00368408379.73597051
ACGGATG5950.00378126749.7053563
CTTGGAT8255.7168538E-59.6548381
CTTTCGT8054.7537222E-48.995191
CTCAAAT9450.0023267417.6625691
CTTAAAG11452.3463047E-47.5889541
CTTATCG9600.00271528267.54284141
CTCCTTT16659.3522067E-77.3933431
CTTTAAT19653.0531737E-87.37010431
CTTCTAT14751.0126665E-57.3638581
CTCAAAA14204.8714996E-57.139141
CTCATCA10450.00620666756.9293091
CCTCCTT11500.00204048476.92629581
CTTTGAT21902.2162567E-76.61290171
TTAGAAT12500.00504578326.353332
TTGCATT12550.00527016966.32684764
TTGTTTA16654.0337085E-46.06949043
CTTAAAA18151.8057172E-45.98440271
Produced by FastQC (version 0.11.5)