FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2656776
Sequences flagged as poor quality0
Sequence length100-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATT7702.3667448E-510.4632551
CACACGT7652.7249418E-510.3304297
CCCTCAT16651.0526492E-88.3580341
CTAAAAC11602.6877497E-58.2082421
CACGTCT9200.00193844347.8077479
CTCAAAC20354.7439244E-87.19830751
CTCACAG11550.00189760996.9755031
CTATTTA11550.00189760996.9755031
CCCACTT13702.1173345E-46.9500451
ACGATTT29452.0008883E-116.8306153
CTTATAT25901.8317223E-96.7869761
CTTCTAA10800.00765759966.78173881
CTTCGAT30252.5465852E-116.77949671
CTTACGA13250.00109733166.63330841
CTTAATA14504.2362654E-46.5665941
CCCAGAT21951.3346162E-66.33992151
CTCCCAT17358.344826E-56.33222871
CTTCTAT25456.3097104E-86.3313991
CTCAAAG26852.7332135E-86.27405551
CTCAAGA16452.8703304E-46.2334281
Produced by FastQC (version 0.11.5)