FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889701
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCAC4300.003834953511.7349455
CCCAGCT7953.1100608E-610.9541921
CCCCGAT5550.002031487410.4607611
CCCACGG6250.00542884939.2891561
TCCGATA7300.0020429978.8873254
ATAGGCG6500.0079280378.8706388
CTCCATT8100.00492334238.0635031
CTTCAGA9750.00309331977.4432341
CTCGATG10650.00780272256.76750568
CTTTGAT12150.0035540776.5702621
CTTCAAG14050.0023534316.19828031
CTTCAGG13200.0084206596.04762741
TTCAAGA15250.00628918555.67492
Produced by FastQC (version 0.11.5)