FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456125
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1700.057.4581957
CACGTCT1850.049.0280349
ACACGTC2000.045.3509338
AGAGCAC2650.034.230873
AGCACAC3000.032.5596435
GCACACG2950.030.7463956
GAGCACA3001.8189894E-1230.2372684
GAAGAGC5704.971298E-915.95107651
AAGAGCA6051.1188604E-815.001912
GGACGCG4450.006308873610.975166
AGTCACG1903.586465E-710.28244420-24
ACGTCTG2356.7352994E-78.90689310-14
CAGTCAC2552.321849E-78.755918520-24
CGTCTGA3404.5838533E-108.61872910-14
CCAGTCA2151.9009241E-58.43775320-24
ACTCCAG3002.720306E-67.442204515-19
GTCACGA2858.333556E-56.85496220-24
CTCCAGT2901.0469222E-46.73647815-19
CTCGTAT2703.2040916E-46.718656535-39
CACGACG3757.2905204E-55.95441625-29
Produced by FastQC (version 0.11.5)