FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905796
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT6001.4852376E-613.4110591
CTCCCGT4300.00343660811.90833951
CACAAAT7953.7905332E-49.2014131
TTAAGAT8558.9324947E-48.4432683
CCCAGCT7950.00387271638.2812721
CTCAATG9050.00137544618.0830091
CTTTAAG9350.00189697377.82366131
CTATTAT10708.52901E-47.52022081
CTTCAGA10950.0010906667.34852551
CTTTCAT14003.5340534E-57.31512361
ATCCGTT10400.0053693867.03377251
ATCACAT10450.0056252877.0001181
TTAAATA12758.323488E-46.79547262
CCACCAT10900.0084592256.7111221
ATTAAAT13200.00106403526.6501121
CTTCAAA14250.0025223916.16010431
CTTTCTT24009.614578E-76.09593631
CTTTGAT15650.0010801916.076461
CTTCTGG16300.00175913955.83414751
CTTTTAT17100.0031102485.5612051
Produced by FastQC (version 0.11.5)