FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2363665
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGAC18606.9795715E-77.01967141
CTCCGAA18703.991926E-56.2063411
GTTAAAT17004.9640494E-45.97360321
ATTAGAA15800.0013434375.9682021
CACCCTT16200.00181180895.8208391
CTCCGTA15900.0093667785.47446061
ATTTGCG21603.416145E-45.3467126
TTGAGAT20409.173584E-45.30784753
CCCGTTT22054.2679824E-45.26342731
CTCCGAT18500.008674955.0971671
ATGTTGG18450.0089015395.08589746
CCACAGG20050.0039948145.06491
CTAAAAT23108.221468E-45.02418041
Produced by FastQC (version 0.11.5)