FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362699
Sequences flagged as poor quality0
Sequence length100-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAC7400.0021769548.826091
CTCTTAC6600.0084980988.7963721
CTCACTT6700.0096108668.6650841
CTACTGT7900.00393407058.2674771
CTCTGAT8700.0093467397.50724941
CTACAAT14556.533337E-56.9826871
CTCCAAA15057.432659E-46.2685121
CTTGGAA17651.1977176E-46.16742851
ACACCAT14350.00318646176.0333928
CTTTAAC17500.00449593975.39091971
CCTTCAT19200.00230916365.29156831
CTTCAAT24958.0420374E-55.2355161
Produced by FastQC (version 0.11.5)