FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313870
Sequences flagged as poor quality0
Sequence length100-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGAG4850.00927230210.42268851
GAGATTG10559.6227566E-58.19613555
CAAAGAT15852.8915001E-56.83416461
CTCCGCT10800.008770796.68651341
CAAGAAT13050.00108293296.64039951
CGAAGAT18854.7775622E-56.1295991
CGCAAAT13050.00788035156.0870331
GTTTCCG21650.00200918645.0033031
Produced by FastQC (version 0.11.5)