FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177229
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAG1700.003219840621.1763881
CGTGAAT6150.0050756519.3658171
CCCTCCT7904.1734404E-49.1138891
CTCAAAT7900.00422325178.2024991
CAACTAT8500.00814677.62351
CTCGAAA9650.00302335747.4611111
CCAAAAG13200.00127452556.54542971
AAGCTTC13200.00134777296.51317935
GTTTGAT12700.00612934356.2361961
TGCTGAC18302.3410819E-45.8722899
CAAGAAT15300.00664579455.64703751
CTGAAAT16900.00326775435.53843981