FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008932

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008932
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592530
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2650.047.513557
CACGTCT3000.041.97039
AGCACAC3200.041.5296025
ACACGTC3700.037.8110778
GAGCACA3350.037.5885164
AGAGCAC4650.027.0798993
GCACACG5200.025.5566796
AAGAGCA8000.018.372862
GAAGAGC8850.015.8535181
AGTCACT2150.013.0122920-24
AAGTTTG4454.3392828E-412.5764314
GTCACTC2407.530616E-1010.49115820-24
ACATTTC6150.00642428879.1000194
CGTATGA3403.974492E-98.229053530-34
TCACTCC3601.1530574E-87.77162225-29
ACGTCTG4152.6993803E-97.415812510-14
CTCGTAT3402.8635623E-77.40589835-39
TCGTATG4451.4442776E-97.229739740-44
CAGTCAC3555.8826663E-77.09261420-24
CCAGTCA3751.9376057E-77.0873620-24
Produced by FastQC (version 0.11.5)