FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences989470
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAG8405.4493976E-610.4528741
TTGTGGG6406.017371E-410.1584777
CTCAAAT8557.544123E-59.4136991
CTCTGCA8456.951435E-48.6591851
CTTTTAA17804.4201443E-108.6324291
CTCAATA7650.0027293648.6082491
GATTTAC9603.049946E-48.2772785
CTTCACT8000.00408916048.2316381
CTTTAAT15602.8125396E-77.97366571
CCCGTTT10255.3742854E-47.8524021
CTCATTT9350.00189254887.82568031
CTTCAAC15151.5411351E-67.72753671
CCCGCTT8650.0082444837.61307571
CTTAAAT9700.0027148577.54331061
CCCATTT9700.0027148577.54331061
TTTGATT20458.5001375E-97.41808942
CTTCTAT12003.574391E-47.31701141
CTTTATT20455.259426E-87.15600161
GTTTGAT11250.00144939547.15441131
CTCTTTT23109.3514245E-96.96858261
Produced by FastQC (version 0.11.5)