FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854015
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATTG2950.0044523214.619283
CTCAGAT5801.6710763E-512.5151551
CTCAAAT7059.8236505E-611.3257711
CTTTAAG5700.00253040610.1877751
CACAAAG8951.2742645E-59.7324551
GTCAGAT6050.00414404049.5983991
CCCACAT6100.00443531539.5197251
ATAAGGG6950.00134135519.3074148
AGATTTA7803.7339254E-49.2151454
CTTCCCT7100.00148927269.20128251
TCCATTG9151.9150703E-48.6410862
CCTCCAC7900.0039222558.2695061
CTTAAAA9050.0014843238.02076151
CAAAGAT16357.937342E-87.99132821
CTTCGTT8350.00645497.82384441
CTCCATT8400.0068096527.77727461
CAACGAT10200.00479227637.1164611
CTTGATT12808.188418E-46.80511571
CAACTAT11750.00249485026.79546261
CTTCAAT15301.2552102E-46.64202931
Produced by FastQC (version 0.11.5)