FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4248700
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAG21101.531771E-87.19732241
CCTTAAT22151.9412328E-66.20317461
CTCGAAT16603.804479E-46.09893661
GTCAAAT28952.6095222E-85.99510531
CCCCATT22802.035056E-55.70915461
CCCAGGG16750.00279685835.61258171
CCAAAAT44051.2732926E-115.58170841
TCGAATT23653.798199E-55.4904212
CAAATTT38808.236202E-85.0322961
Produced by FastQC (version 0.11.5)