FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787248
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGAT2951.8982284E-417.459231
CCCCTCC4251.9202962E-413.8500111
CTCAGTT3750.0011756813.7345931
CTTGAAT8456.2370855E-59.5782251
CTCCGGA6500.00669053669.0557771
GTTCATT9051.3183922E-48.9432061
CTCAAAG9251.671681E-48.7498381
CTATGAG6750.0091162568.7203781
GTAAGAA8150.00459564158.1251981
CTTCAAT13451.9214653E-57.6586961
CAACAAT9950.0032960867.3947931
CTTCAAA15051.0733582E-57.33337351
CATAAAT10450.0053143377.0409751
TAAATTA10550.0070192096.8417774
CAACAAA13850.00171470366.37500521
CCTCTTT13400.00852794856.04000041
ATTGATT13300.0096098275.9705952
CTTTCTT20851.5621404E-45.6462881
CTTCTTT25001.1187867E-55.59194231
CATCAAT15950.008269065.5356631
Produced by FastQC (version 0.11.5)