FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628749
Sequences flagged as poor quality0
Sequence length100-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT2508.56071E-825.8576589
ACACGTC2701.8021456E-723.9422768
CACACGT2802.5601003E-723.0871947
AGCACAC4206.543505E-717.1016255
GAGCACA4903.2549706E-614.6620344
GCACACG5054.4606986E-614.2231346
AGAGCAC5551.1797769E-512.9448593
TTCGATC4700.0076143810.7018682
CTCAAAA6400.00697247269.0098811
AAGAGCA9250.00203252587.7681512
CTGCAGG8400.00747820047.6957316
CAAAAAT10709.969753E-47.4099961
CGTCTGA4051.458391E-45.675205710-14
CACACAT3600.006953365.194846625-29
Produced by FastQC (version 0.11.5)