FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125053
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT10700.041.741317
ACACGTC11050.039.777628
CACGTCT10900.039.674619
GCACACG13650.031.6815556
AGAGCAC14200.029.4573353
AGCACAC15400.028.0820395
GAGCACA15550.027.3552324
AACTAGA2000.001092049625.50359145
GAAGAGC26150.017.1457821
AAGAGCA25900.016.448862
AGTCACA11900.08.10380820-24
CAGTCAC12700.07.816665620-24
AGCTACG10005.795429E-47.7983377
ACGTCTG14100.07.24013410-14
CTCTCAT11000.00153458787.11686041
TCTCGTA12000.06.9721635-39
GAGATCT11700.06.908885530-34
GTGGGAT11350.00213651456.89739751
AGATCTC12250.06.5983535-39
ACTCCAG15300.06.394569415-19
Produced by FastQC (version 0.11.5)