FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1343479
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTGGA14501.4973557E-67.7430811
CCTTCGT9200.00243685257.6273561
CCCATGT8400.0092236897.5183931
CTACAAG10450.00103346997.38649231
CACCAAG9800.0045179717.16037461
ATTTCGC10900.00171097657.0435936
CTCAAAT10400.0080378246.74727631
CTTTTAA21251.5919541E-66.27417331
CTTTGGT13950.00316938136.03627351
CTTCGAT14000.00329862166.01471471
CTCAAAA16706.034683E-45.88265561
TCGATTT15950.00237755065.6890933
CCCGGAT15100.00763621235.57655671
CTTTCCG24401.6538252E-55.46418761
CTTTTGA21554.923806E-45.2099621
GAAATGC20200.00125901745.18284135
CTTATTT21755.6097325E-45.16205451
CTTTATT27402.0672485E-55.12202021
Produced by FastQC (version 0.11.5)