FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4204728
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT16808.442694E-66.8981791
CTCAAAC27907.5211574E-85.9710041
CTCAGAA19658.382184E-55.89767931
TTTAGCC15500.00746097465.5890127
CTTTAAT46254.9112714E-115.32464841
CCCACTT20700.00106647535.24861431
CTTTAAA44254.4019544E-105.23793941
CTTCGAA31901.1073134E-65.2222891
CTTACAT20900.00121227955.1983881
CTTCAAG53503.6379788E-125.14462331
CTCAGGA21300.00155960425.10076571
CTTAAAA58350.05.08940171
CTTCCAT54901.0913936E-115.01343061
Produced by FastQC (version 0.11.5)