FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences929071
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATAG4502.0234938E-514.6132491
CTCGTAT4906.775508E-411.9291831
CTCATGG6809.453949E-49.6705321
CCTTGGT7250.0016974189.0702921
CACCGCT8658.894061E-48.4469641
CTTCGAT10709.637544E-58.1943451
CTAAAAT10406.3740695E-47.728161
TCGATTT9400.00227835337.6792583
TTCGATT8500.0079777237.6410642
CATCCAC11602.4585624E-47.55857661
CTTCAAA17501.7825605E-67.0978641
CTTTCAA14454.1730335E-46.5734341
ATCAGCT12250.0036070376.56105041
CGATTTC13450.00152947636.44034
CATCAAT15107.1167445E-46.29047161
CTTCAAT14050.00218084696.24053341
CTCTTTT20952.9179395E-55.9290031
CAACAAA15300.00564429165.73068571
AAGATAA20057.258792E-45.40037635
Produced by FastQC (version 0.11.5)