FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919184
Sequences flagged as poor quality0
Sequence length100-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTGG5058.6143665E-411.5926671
CTCCAAT8808.079751E-710.8104921
CTCAAAC6750.0095340558.6730321
CTCAAAT9402.1120303E-48.5634661
CTTGTTT18509.404175E-108.3067721
CTTCAAT18659.566065E-97.84758331
CCTGAAT11953.4015087E-47.34848931
CCTTGAT12053.7433466E-47.28750661
CTTCTCT13201.351381E-47.2069941
CTTCAAC12300.00370392796.54443741
CTTTTAT26904.1891326E-96.52895551
CTCTTTT23651.069493E-76.49789861
CTCAAAA13150.0074278676.12141281
CTGAAAG16100.00151101095.90883971
CTCCTTT18902.7175286E-45.80783371
CTTTATT26104.237936E-65.6075641
CTTCCTT22901.10151144E-45.4324811
CTTTAAT17750.00480997145.3595671
ATTAAAT25601.0547998E-45.1453781
CAAATTC18500.0090474415.0779183