FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220049
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAAAG4700.00737028110.7502461
TCTACGC8100.00535222057.98942768
CCTCGTT11954.671111E-57.8522421
CAACAAA15703.8764847E-77.8156931
CCCTGTT10256.22153E-47.746171
CCTCAAA12155.7437694E-57.7229861
GATTTAA8450.0078231137.6581915
CAACTGT8650.0093139587.51008131
CCCAGAA10850.00114116777.31781051
CTTCTAC10900.0096280666.6220391
ATAATCG12250.00426958556.4564973
TTCACGC14907.3584536E-46.27357777
AACAAAC12850.00701079756.15603642
CTTTTGA14200.0028189286.0997371
CTTTCAC14300.00304914526.0570811
CTTTGGA14350.00317045466.03597641
ATCAGAT15650.00126947745.9958011
CTTCAAT18302.1142559E-45.9164121
CTTCATT14700.0041479735.89226251
TTTTATG17206.4397365E-45.85272037