FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005008997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005008997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084513
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAT4907.142826E-411.8547851
CTTGTGT6354.2140426E-511.4347331
CCTCCCT4450.004694004511.42188551
CCCAACA5250.001273008211.0644661
CATAGCT6656.7434965E-510.918881
CTCATAC7802.9977953E-510.2399511
CACAGTT7251.6176993E-410.015251
CTTGCTT10457.6489414E-79.727731
CCCACAT6000.003861019.6814081
CTACCAG7100.00148573459.2041561
CTTCCGA10508.528985E-68.9898791
CCCATGT6500.00746238938.9366851
CTATAAG8507.95782E-48.5424191
CTCAATC8700.00100236728.3460421
CCAATCT7800.00362761958.3418073
CTTTGAT19703.7343852E-97.7402121
CTCCTGA10709.235224E-47.46463731
CTATAAT13853.3833585E-57.3396961
CCCATTT9950.0037538427.29754351
CTTAAAT14204.7039874E-57.15878771
Produced by FastQC (version 0.11.5)