FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences778833
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAC3850.001514614113.2809531
CTTGAAC5500.001784343710.6247631
CTCAAAG8506.399523E-610.312271
CTAAATT5800.002774218810.0752071
CTTCGGT8104.6346552E-49.0179311
CATTAAT9452.2802422E-48.5026221
CTCCCAA7850.00349893038.3746141
CTTCAAC10154.9238716E-47.91623351
CTTCGAT8500.00715542267.73420241
CTTTCGG10658.2360767E-47.54457951
TCCGATT8650.0093473647.50639342
CTTGAAT9900.00336871927.37830731
CTTCAAA16354.946738E-67.14815861
CAAGAAT13501.821374E-47.03398661
CTGGAAA16400.0019242795.79017161
CTTTTCA19303.6980145E-45.67709161
TTTCCGT17050.00354031825.5004227
CTTTATT20407.8029186E-45.37097361
CTTTTAT18150.00637932085.2318911
Produced by FastQC (version 0.11.5)