FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971927
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAAC5559.887623E-613.1703821
TTAACCT5350.001555242210.8019593
CTTTTAG5650.002220076510.3498211
CCCGATT5750.002568250310.1698251
CTCCAAT7552.2755009E-49.6815391
CCCTCAT6809.4200537E-49.6744191
CTTCAGT7602.4316853E-49.6178451
CGAAAGA9903.8641705E-58.8600751
AAATAGG6600.0088305858.7547835
CTCAAAA13402.2706263E-68.1823441
CCCCCCC1650.00601944377.88652645-49
GTTTCGG10950.00124868617.25567536
CAAATTT11200.00158361477.0948143
CTCAACA11600.00202542456.9314811
CTTTTGA14500.00309144036.0492921
AAGTTTT13300.00954933655.974583
GAATTTT15950.00157744945.8877454
CTTGAAA15350.00582677035.7143151
CTGAAAG16850.002636155.63942431
Produced by FastQC (version 0.11.5)