FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729206
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATAA5150.00110934611.2472021
CTTCCAC10457.9373604E-79.700041
CCCGGAT8306.112589E-59.5956921
CAACCAT6150.00484315179.4183881
GTTTGGC6550.0082624728.8256316
CTCTTTA6600.0086511628.7762261
CCATAAT8400.0070848667.74265862
CTTGAAA12407.105498E-57.59072731
CCTGCAT12004.0295E-47.24038651
CGATTTT10200.0050027167.08478554
CTTCAAT11350.0017776697.0171141
AGATTTT15051.0722237E-46.7223084
CAATGAA13050.00104935966.65782641
CTTCCTT13250.00124672136.5573311
CTTTCTT18002.335488E-56.43589831
CATCAAT13650.00174439096.3651751
CAAAAAC12950.00707150586.15013461
CTTCCGG13150.0082878026.05659681
CTTTTAT14500.00343466465.99204351
CAAAAGA15050.0051975345.7730651
Produced by FastQC (version 0.11.5)