FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences988561
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCT6859.660798E-510.5387613
CCCAAAT6300.00548225279.2782941
CCCACAA6350.00585068069.2052361
CACAGAG6700.0090857798.7243661
CCCTTTT11753.452551E-68.7058031
CATTAAT7600.00260514188.6526191
CTTTTAA15151.8000537E-78.1988891
CTTTTGA14356.0513958E-67.63761951
CTCAAAA12706.9516845E-46.90392731
CTCCATT10700.007154466.82865051
CTTTCGC11000.009343466.64241461
CCAAAGA12300.00376456566.53440861
CTTCAAA17951.9646059E-56.51289751
AGATTTT19901.5946955E-56.16702944
CTTCATT16904.1819012E-46.05285171
CTTCAAT16904.1819012E-46.05285171
CATCAAG16300.0017841845.82739451
GCAGGGC15100.0055770965.73699478
TCCATTT17759.144993E-45.6936122
GAAGATT15500.00744630355.5889444
Produced by FastQC (version 0.11.5)