FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316669
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAATC5451.3859154E-411.904492
CCCCCTT4907.305192E-411.8234441
CCCTTCG7250.0018417888.9898951
CTCTATT10508.82488E-68.9661121
CCCGTAT8205.7157106E-48.8315361
CCTCATT11753.897845E-68.6285981
TTAATCT9050.0015933927.96404553
CTCAGAA10400.00592470266.9633271
CTCTTAT10550.0068064026.8643221
CTTTCAC11700.0024472416.8085861
TTTAAGC10700.0081691346.7359457
CTCAAAT11900.00292450146.69415571
CTCCTTT22957.395829E-86.62653831
CAATCAT15057.622047E-46.25542741
CTATAAT13900.00213843636.25196551
CTTCAAA24101.2430373E-66.00984241
CAATGAT18201.8760705E-45.96856551
CTGATAT18652.617105E-45.82455251
GATTCTG17356.983196E-45.8158195
AAGATCG15050.00547619685.7468135
Produced by FastQC (version 0.11.5)