FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1226322
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAAT6505.0113307E-511.2423161
CTTTAGT5650.002225992310.346911
CTAAATC7402.1055226E-49.7570082
AGATCTC6150.00495670369.3928784
GGTACAT8457.0450036E-48.6479361
CAATCGG8357.0476043E-48.64763454
CTTCCGT13051.5626538E-68.3994321
CCTTCCT11953.4602408E-47.33808141
CTTGTAT13852.4849293E-46.85903071
CTTTAAT19501.345581E-66.74539041
CTTCAAT19056.725608E-66.5211341
CTCATCC13900.00193175626.30863861
CTTAAAT13950.0020113816.2860271
TTCAATC17306.5791875E-45.8429252
CTTGTAA15050.0046985855.8265831
CTTTGAT21805.3504715E-55.6985141
CTTTAAA19504.236406E-45.62115861
GTTCCAT18750.00156960525.4562711
CTTTTAA25701.9985138E-55.4024361
CCTGTTT20307.279666E-45.3996351
Produced by FastQC (version 0.11.5)