FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709104
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAAA15552.910383E-119.90409851
CCCACGA8658.5821445E-48.4783221
CACAGAG12406.794098E-68.2800391
CCCAGAC12053.656804E-47.30331851
CTGACAC11150.00128831137.2350891
CTTTAAA20152.969282E-76.91519741
CCCCATT10650.0066026846.8861491
CTCCCAA10750.007227646.8220921
CCCAGTT12050.00292317036.69470831
CACAGCT13400.00122771526.56753641
CACCCAT12350.00378254436.5320841
CCCCGAA13500.00133545816.5188881
ACAGATC12350.0046015676.41069843
CCCAGCT16202.3137519E-46.33780771
CAACAAA25959.000905E-86.21743631
CCCACAT13250.00786523156.0883951
CATTAAT14650.00334662576.0071661
CTTCAAT28207.233939E-85.98142621
AAGTTGC13250.0095482265.97525455
CTCATTT16350.00177307475.83111521
Produced by FastQC (version 0.11.5)