FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783045
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATT4500.00539405511.210232
GGTAGAA6304.521857E-410.4778521
GATTACG5650.00249816910.203357
CCAAAAG7650.00266965328.62881851
CTCAAAA10951.2055345E-48.0378041
TCAAACT8200.00585222437.9096572
CTCTTTT18153.8418693E-77.2738811
CCTTCAT11400.00162484997.07714561
GGATGAT11650.0020433376.92527531
AAGTTTT11550.00224772756.86296845
CAGGAAT10900.0082429636.72889571
CGATGAA12700.0050500736.35271361
CTTTCAT12850.00570669076.2785571
CTGCTTT17355.573905E-45.91832541
ATTAAAA16350.00176691145.83170941
AGAAATA17000.00346414955.51055964
Produced by FastQC (version 0.11.5)