FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005009058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005009058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446886
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1850.049.934987
ACACGTC2350.042.33448
CACGTCT2050.041.596879
AGCACAC3201.8189894E-1228.868665
GCACACG3201.8189894E-1228.868666
GAGCACA3600.027.6349564
AGAGCAC4850.021.9777283
GAAGAGC6206.657501E-1016.1164341
AAGAGCA7355.020411E-1014.502312
CTCATGC3300.009791370512.9768691
GTTATAT4756.3428306E-412.0206791
CAGTCAC3053.6379788E-119.79952820-24
CGTCTTA2057.8381345E-79.71180430-34
AGTCACC2755.857146E-99.31591620-24
GTCACCT2951.9466825E-88.68432820-24
CTCGTAT3156.548362E-98.58265735-39
CCAGTCA3052.5914887E-67.46630720-24
ACTCCAG2951.320085E-57.22835415-19
TCGTATG3052.0960835E-56.9940240-44
CTCCAGT3351.0667047E-56.78961815-19
Produced by FastQC (version 0.11.5)